16 KEY INGREDIENTS the fruit as xanthones. Abundant from Fytexia, France. The supple- bioactive xanthones found in the ment is produced by hydro-alco- fruit are α- and γ-mangostins (9) holic extraction of whole fruit and , for which antioxidant (10)and an- contains 10% of a 5-10:1 ratio ti-inflammatory (11)properties have of α- and γ-mangostins.For the been reported in the literature. In NSAID studied in the clinical in- the present study, we assessed a vestigation,nimesulide was pur- dietary supplement obtained by a chased from a local drugstore in traditional extraction process of France. In the preclinical study, mangosteen, whereby the repre- inflammation was induced using sentative, original fingerprint of lipopolysaccharides (LPS) from bioactive xanthones in the fruit are Escherichia coli serotype 055:B5, mainly α- and γ-mangostins in a ra- purchased from Sigma (St. Louis, tio of 5-10:1.Taking a mechanis- MO, USA). tic approach, an in vivo pre-clinical model of inflammation was used Pre-clinical model to evaluate supplement efficacy of inflammation on the release of TNF-α. Salutary Animals effects on pain management have Eighteen male CD-1 mice (Charles been assessed and compared to River Italia,Calco, Lecco, Italy), an NSAID reference in a clinical in- weighing /home/webapps/asp_fr/data/asp/publications/gate-to-business-(groupe-bgm)/fevrier-(1)25 g each, were ran- vestigation of volunteers suffering domly divided into three groups from acute or chronic soft tissue of six animals. They were housed inflammation. at 23 ± 1°C with relative humidity materialandmethods 60%, subjected to a 12 h/12 h light/dark cycle, and had free ac- cess to food and water. Animals were handled in compliance with national and international laws and policies.The control group was Materials treated with LPS alone (LPS). MangoSelect, an extract of Man-® The supplemented group was gosteen prepared by traditional treated with LPS + mangosteen extraction methods, was obtained extract (MGS), whereas the stan-