COMUNICACIONES PÓSTERS Nº ovocitos Nº ovocitos Valor predicho Límite de Conf. Límite de Conf. Edad donante recuperados maduros de embarazo al Inferior (95%) Superior (95%) asignados finalizar el ciclo 18-29 <20 ≥8 0.7422 0.5995 0.8471 18-29 ≥20 ≥8 0.6082 0.4551 0.7425 30-35 <20 ≥8 0.5723 0.3789 0.7458 18-29 <20 5-7 0.5671 0.4461 0.6806 18-29 <20 3-4 0.5063 0.3106 0.7001 30-35 ≥20 ≥8 0.4190 0.2524 0.606 18-29 ≥20 5-7 0.4139 0.2817 0.5597 30-35 <20 5-7 0.3784 0.2328 0.5498 18-29 ≥20 3-4 0.3560 0.1786 0.5843 30-35 <20 3-4 0.3228 0.1590 0.5458 30-35 ≥20 5-7 0.2470 0.1316 0.4154 30-35 ≥20 3-4 0.2044 0.0843 0.4175 P-003 CHARACTERIZATION OF OVARIAN FOLLICULAR RESUMPTION AFTER DRUG-FREE IVA IN PRIMARY OVARIAN INSUFFICIENCY PATIENTS M. Méndez Justo, JM. Calafell Pozo, J. Ferreri Dos Anjos, S. Cívico Vallejos, D. Manau Trullàs, F. Fabregues Gasol. Hospital Clínic de Barcelona - Barcelona (Barcelona) INTRODUCTION: PTEN inhibitors in mice and human has been recently develo- ped. Other studies suggested that ovarian fragmentation could Primary ovarian insufficiency (POI) occurs when the ovaries stop interfere with the ovarian Hippo signaling pathway, resulting in functioning normally and patients start to have menopausal ovarian follicle growth. Kawamura et al. (2) combined these two symptoms before age 40. Despite having a few remaining folli- methods in an in vitro activation (IVA) strategy to treat infertility cles, their only chance for bearing a baby nowadays is through in patients with POI. egg donation. Furthermore, it has been proposed that the IVA approach could Distinct studies have been focusing on the determinants and be used in patients with diminishing ovarian reserve and early intracellular signaling pathways involved in the activation of pri- stage of POI. As these patients have spontaneous activation of mordial follicles, as the maintenance of a correct ovarian reser- dormant primordial follicles reaching the secondary stage, se- ve will depend on a balance between activating and inhibiting condary follicle growth could be promoted in these patients factors (1). using drug-free IVA without tissue culture. For patients whose ovaries still contain residual secondary follicles, it is likely that, A method for the activation of dormant follicles using in vitro the fragmentation step alone, which implies an Hippo signaling culture of ovarian fragments treated with PI3K stimulators and pathway disruption, is sufficient to promote follicle growth (3). 123 ASEBIR. Revista de Embriología Clínica y Biología de la Reproducción. Noviembre 2021 Vol. 26 Nº 2